4/7/2023 0 Comments Heatmap in graphpadThe intrinsic activity introduces a baseline velocity and is complicating our kinetic analyses a bit. Complicating it even further is a noted double substrate inhibition pattern (apparently not uncommon with ping-pong mechanism enzymes). Of course, upon addition of substrate, the velocity of the reaction increases and NADH is consumed more rapidly. ![]() ![]() I’m working with a rather complicated ping pong, bi-bi double displacement reductase enzyme that oxidizes NADH and then reduces a substrate. Interestingly, the enzyme has intrinsic NADH oxidase activity in the absence of the substrate.
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